Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Reexamination Certificate
2006-04-25
2006-04-25
Wax, Robert A. (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
C530S412000, C435S069100, C436S006000
Reexamination Certificate
active
07034119
ABSTRACT:
A method for the recombinant production of a polypeptide by expressing a nucleic acid encoding said polypeptide in a microbial host cell, forming in the cytoplasm of said host cell inclusion bodies containing said polypeptide, and isolating, solubilizing and naturing said polypeptide, characterized in that after fermentation the host cell or the host cell content is incubated at a temperature of 40° C. or higher for at least 10 minutes and subsequently insoluble polypeptide is isolated from the host cell, this method providing an improved yield in inclusion bodies containing the desired polypeptide.
REFERENCES:
Chrunyk et al., “Inclusion Body Formation and Protein Stability in Sequence Variants of Interleukin-1B,” Journal of Biological Chemistry, (Aug. 25, 1993) 268(24) 18053-61.
TAng et al., “Production of Human Proinsulin inE.coliin Non-Fusion Form,” Biotechnology Letters, (Jul. 1993) 15 (7) 661-666.
Chen et al., “Production of Human Insulin in anE.coliSystem with Met-Lys-Human Proinsulin as the Expressed Precursor,” Applied Biochemistry and Biotechnology, (1995) 55(1) 5-15.
Boye et al., “The Role of dam Methyltransferase in the Control of DNA Replication inE.coli,” Cell, (1990) 62 (5): p. 981-989.
Gruenbeck Rainer
Kopetzki Erhard
Popp Friedrich
Hoffmann-La Roche Inc.
Johnston George W.
Rooke Agnes
Tramaloni Dennis P.
Wax Robert A.
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