Method for rapid identification of prokaryotic and eukaryotic or

Details Method for rapid identification of prokaryotic and eukaryotic or Method for rapid identification of prokaryotic and eukaryotic or

1996-10-29

1998-12-15

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, C12Q 168, C07H 2102

Patent

active

058494928

ABSTRACT:
A method is described for retrieval of phylogenetically informative DNA sequences which comprise searching for a highly divergent segment of genomic DNA surrounded by two highly conserved segments, designing the universal primers for PCR amplification of the highly divergent region, and amplifying the genomic DNA by PCR technique using universal primers.

REFERENCES:
Lane et al, PNAS 82:6955-6959, 1985.
Bottger, FEMS Microbiol. Letters 65:171-176, 1989.
Naito et al, J. Forensic Sciences 37:396-403, 1992.
Schneider et al, Nucleic Acid Res 18:6097-6100, 1990.

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