Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-03-07
1995-04-11
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 436 94, C12Q 168, C12P 1934, G01N 3348
Patent
active
054057477
ABSTRACT:
Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.
REFERENCES:
patent: 4962037 (1990-10-01), Jett et al.
patent: 4962045 (1990-10-01), Picozza et al.
L. M. Smith et al., "Fluorescence Detection in Automated DNA Sequence Anais," Nature 321, 674 (1986).
James M. Prober et al., "A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides," Science 238, 336 (1987).
James H. Jett et al., "High-Speed DNA Sequencing: An Approach Based Upon Fluorescence Detection of Single Molecules," J. Biomolecular Struc. & Dynamics 7, 301 (1989).
Harding et al., Trends in Biotech. 10, 55.57 (1992).
Hammond Mark L.
Jett James H.
Keller Richard A.
Marrone Babetta L.
Martin John C.
Freund Samuel M.
Horlick Kenneth R.
Parr Margaret
The Regents of the University of California Office of Technology
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