Method for rapid base sequencing in DNA and RNA

Details Method for rapid base sequencing in DNA and RNA Method for rapid base sequencing in DNA and RNA

1987-10-07

1990-10-09

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 41, 435 75, 436501, 436 94, 436175, 436800, 935 77, 935 78, C12Q 168

Patent

active

049620371

ABSTRACT:
A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.

REFERENCES:
patent: 3730844 (1973-05-01), Gilham et al.
patent: 4521509 (1985-06-01), Benkovic et al.
Baumlein et al., (1986) Nucleic Acids Research 14(6):2707-2720.
Shimkus et al., DNA, 5:247 (1986).

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