Method for rapid analysis of hemoglobin variants by high speed l

Details Method for rapid analysis of hemoglobin variants by high speed l Method for rapid analysis of hemoglobin variants by high speed l

1977-05-18

1978-08-22

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

210 31C, 424101, G01N 3108, G01N 3316

Patent

active

041086031

ABSTRACT:
A method for analysis of hemoglobin variants by high speed liquid chromatography and, more particularly, by ion-exchange chromatography. Through the use of high flow rates and pressures, the hemoglobin variants can be rapidly forced through the separation column to thus enable rapid analysis. A bonded phase inorganic support column is utilized for separation of the hemoglobin variants with the profiles thereof then being obtained. The bonded phase support preferably includes ion-exchange groups bonded through a carbohydrate intermediate to controlled porosity glass. A pair of different columns may be utilized to separate the hemoglobin variants with one of the columns being an anion-exchanger, and more particularly, a diethylaminoethanol glycophase, controlled porosity glass column, with the other column being a cation-exchanger, and more particularly, a carboxymethyl glycophase, controlled porosity glass column. The hemoglobin profiles obtained from such a pair of columns are different and the combination of chromatographic data obtained identify the particular hemoglobin variants and can thus be utilized for diagnosis purposes.

REFERENCES:
chemical Abstracts, 83:55371r, (1975).
S. Chang et al., Anal. Chem., 48(13), 1839-1845 (1976).

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