Method for radiolabeling proteins with technetium-99m

Details Method for radiolabeling proteins with technetium-99m Method for radiolabeling proteins with technetium-99m

1981-03-23

1984-01-03

Nucker, Christine M.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

424 9, A61K 4300, A61K 4900

Patent

active

044242000

ABSTRACT:
Proteins are radiolabeled with technetium-99m in a reducing environment comprising buffered stannous chloride wherein the tin utilized to form the stannous chloride is non-oxidized, the buffered stannous chloride is purged of oxygen and the buffer comprises an alkali metal biphthalate and an alkali metal tartrate.
Proteins which have been preincubated with tin are radiolabeled with technetium-99m to form a strongly bonded, non-exchangeable radiolabeled tracer substance. The radiolabeling can be accomplished immediately after pretinning, or the pretinned proteins can be freeze-dried and the radiolabeling accomplished at some time in the future when the pretinned proteins are resolubilized in a saline solution of sodium pertechnetate - TC-99m. Pretinning can be accomplished in a reducing environment comprising stannous chloride buffered with an alkali metal biphthalate and an alkali metal tartrate, this solution having been purged of oxygen to assure that the stannous chloride is non-oxidized. When the pretinning proteins are freeze-dried, they can be utilized to prepare instant TC-99m labeling kits for use as radiopharmaceuticals.

REFERENCES:
patent: 3872226 (1975-03-01), Haney et al.
patent: 4042677 (1977-08-01), Molinski et al.
patent: 4070493 (1978-01-01), Nadeau
patent: 4247534 (1981-01-01), Bevan
McManus et al, Can. Res., 36, pp. 2367-3481, Sep. 1976.
Wong et al, Int. J. Appl. Rad. Isotopes, 29, 251-253, 1978.

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