Method for quantitatively determining the expression of a gene

Details Method for quantitatively determining the expression of a gene Method for quantitatively determining the expression of a gene

1998-04-06

2000-07-18

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 9121, 536 231, 536 232, 536 235, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

060905565

ABSTRACT:
The present invention relates to a method for quantitatively determining the expression of a gene, comprising providing at least two types of samples each containing a cDNA coding for the gene, adding a different adaptor to each of the cDNAs contained in the samples, mixing equal amounts of the samples each containing the adaptor-tagged cDNA, amplifying the resultant cDNAs and calculating an amount ratio between the amplified products.

REFERENCES:
patent: 5707807 (1998-01-01), Kato
patent: 5814445 (1998-09-01), Belyavsky et al.
Kato, K., Adaptor-tagged comptetitive PCR: a novel method for measuring relative gene expression:, Nucleic Acid Research, 25:4694-4696 No. 2 (Nov. 1997).
Kato NAR vol. 24, No. 2 pp. 394-5, 1996.
Kato NAR vol. 23, No. 18 pp. 3685-3690, 1995.

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