Method for quantitatively determining the degree of agglutinatio

Chemistry: analytical and immunological testing – Involving kinetic measurement of antigen-antibody reaction

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422 73, 422 82, 436509, 436514, 436524, 436534, 436805, 73 641, G01N 3354, G01N 3508, G01N 2185, G01N 2103

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active

043988946

ABSTRACT:
A method and apparatus for quantitatively determining a degree of agglutination of a suspension of particles or the presence of the agglutination thereof is disclosed, wherein a liquid which contains agglutinated clots, otherwise a substance or substances to be about to agglutinate is made to slowly transfer through a small tube, in course of which agglutinated clots and non-agglutinated particles separate from each other in the liquid, when a degree of agglutination of particles and the concentration of the substance to be tested herewith can be determined quantitatively through the detection of difference in the optical properties of both the accumulation layer of agglutinated clots and the suspension layer of non-agglutinated particles, or the change in the optical properties of either of the above two layers, in particular the suspension layer.

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patent: 4253846 (1981-03-01), Smythe

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