Method for quantitatively determining the amount of hemoglobin i

Details Method for quantitatively determining the amount of hemoglobin i Method for quantitatively determining the amount of hemoglobin i

1982-09-13

1986-01-28

Turk, Arnold

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

436 97, 436172, 436178, G01N 3372

Patent

active

045671486

ABSTRACT:
A method of quantitatively determining the amount of hemoglobin in a biological material which includes the steps of preparing a test sample of the biological material by removing contaminating elements such as sources of interfering fluorescence, converting the hemoglobin in the test sample to porphyrin, performing an extraction step for the purpose of separating the porphyrins derived from hemoglobin from various contaminants and determining the level of porphyrin in the test sample by means such as fluorescence assay.

REFERENCES:
patent: 3874853 (1975-04-01), Byrnes
patent: 4035150 (1977-07-01), Jaffe
patent: 4378971 (1983-04-01), Schwartz
Henry et al., Clinical Chemistry-Principles and Techniques, 2nd Edition, Harper & Row, New York, 1974, pp. 1215-1263.
Morrison, Analytical Chemistry, vol. 37, No. 9, Aug. 1965, pp. 1124-1126.

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