Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2007-04-03
2007-04-03
Navarro, Mark (Department: 1645)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C435S183000, C530S350000
Reexamination Certificate
active
10755236
ABSTRACT:
The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.
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Ebinuma Hiroyuki
Matsumoto Takuji
Sarashina Takami
Sekiguchi Masahiro
Ushizawa Koji
Daiichi Pure Chemicals Co. Ltd.
Navarro Mark
Oblon & Spivak, McClelland, Maier & Neustadt P.C.
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