Method for quantitatively determining glucoamylase in human urin

Details Method for quantitatively determining glucoamylase in human urin Method for quantitatively determining glucoamylase in human urin

1976-01-26

1977-02-22

Monacell, A. Louis

Textiles: fiber preparation

1951035C, G01H 3114

Patent

active

040090795

ABSTRACT:
A method for quantitatively determining glucoamylase in human urine and body fluids which comprises removing low molecular weight substances from a human urine or body fluid sample, adding to the resulting sample which is free of low molecular weight (from about 500 to about 20,000) substances maltotriitol, phenyl-.alpha.-glucoside or 2,4-dinitrophenyl-.alpha.-glucoside, which are specific substrates for glucoamylase, and quantitatively determining the glucose, phenol or 2,4-dinitrophenol which is a degradation product of the above substrates, respectively.

REFERENCES:
chem. Abstracts, vol. 79, 1973, 50268v, p. 114, "Kinetic Studies of Glucoamylase".

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