Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol
Reexamination Certificate
2003-10-16
2010-12-14
Hobbs, Lisa J (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving cholesterol
C435S019000, C436S071000
Reexamination Certificate
active
07851174
ABSTRACT:
A method for quantitatively determining cholesterol in high-density lipoprotein in a sample, which comprises: reacting a sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, an oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising i) nonionic surfactant, polyanion and albumin or ii) a combination of a surfactant selected from the group consisting of polyoxyethylene alkylamine or polyoxyethylene alkenylamine and a surfactant selected from the group consisting of polyoxyethylene polycyclic phenyl ether sulfate and an anionic bile acid derivative, and measuring the formed hydrogen peroxide or a reduced coenzyme; and a reagent used therefor.
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U.S. Appl. No. 10/531,316, filed Apr. 13, 2005, Katayama, et al.
Fujinaka Mayumi
Katayama Yuki
Fitzpatrick ,Cella, Harper & Scinto
Hobbs Lisa J
Kyowa Medex Co., Ltd.
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