Method for quantitatively determining a particular substrate cat

Details Method for quantitatively determining a particular substrate cat Method for quantitatively determining a particular substrate cat

1982-05-13

1983-09-13

Kaplan, G. L.

Chemistry: electrical and wave energy

Processes and products

204403, 435190, 435817, G01N 2754, C12N 904

Patent

active

044040660

ABSTRACT:
A method for control of relative enzyme activity for analytical purposes. The activity of a multisubstrate enzyme may be controlled as a function of the electrical potential applied to the enzyme. The enzyme is preferrably incorporated into a thin layer electrochemical cell laminate having exterior membrane layers and an interior enzyme layer. A control electrode located within the enzyme layer applies an electrical potential to the enzyme. An intermediate electron transfer agent may be used to transfer electrons to and from the enzyme and the control electrode.

REFERENCES:
patent: 3919052 (1975-11-01), Fresnel et al.
patent: 4016044 (1977-04-01), Fresnel et al.
patent: 4073713 (1978-02-01), Newman
patent: 4129478 (1978-12-01), Racine et al.
patent: 4220503 (1980-09-01), Johnson
patent: 4224125 (1980-09-01), Nakamura et al.
G. A. Hamilton et al., "Oxidase and Related Redox Systems", 103, (1965).
Paul J. Taylor et al., Anal. Chem., vol. 49, No. 6, pp. 789-794, (1977).

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