Chemistry: analytical and immunological testing – Involving diffusion or migration of antigen or antibody – Through a gel
Reexamination Certificate
2006-12-26
2006-12-26
Chin, Christopher L. (Department: 1641)
Chemistry: analytical and immunological testing
Involving diffusion or migration of antigen or antibody
Through a gel
C435S007100, C435S069100, C435S070210, C435S328000, C436S512000, C436S517000, C436S538000, C436S548000, C436S166000, C436S172000, C530S387300, C530S391100, C530S391300, C530S391500, C536S023530
Reexamination Certificate
active
07153701
ABSTRACT:
A method for quantitatively detecting an antigen which comprises (1) a first step of providing an Fab′ antibody having a uniform isoelectric point, said antibody forming an immune complex with an antigen in an analytical sample and being modified by adding an amino acid sequence comprising a charged amino acid residue and by being labeled with a fluorescent dye, (2) a second step of mixing the Fab′ antibody having a uniform isoelectric point with the analytical sample containing the antigen to obtain a mixture comprising the immune complex, (3) a third step of separating the mixture by performing electrophoresis in a carrier, (4) a fourth step of irradiating an excitation light which excites the fluorescent dye to the mixture separated in the third step to cause fluorescence in the immune complex, and (5) a fifth step of detecting the fluorescence.
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Kiyohito Shimura, “Affinity Probe Capillary Electrophoresis: Antigen Quantification and Analysis of Specific Interaction.”, Japanese Journal of Electrophoresis, 1995, vol. 39, No. 6, pp. 349-353. Abstract only.
Hisada Sunao
Ito Yukiko
Kasai Kenichi
Matsumoto Hiroyuki
Shimura Kiyohito
Chin Christopher L.
Grun James L.
Hamamatsu Photonics K.K.
Leydig & Voit & Mayer
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