Method for quantitative measurement of gene expression using mul

Details Method for quantitative measurement of gene expression using mul Method for quantitative measurement of gene expression using mul

1993-04-06

1997-07-01

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 935 77, 935 78, C12P 1934, C12Q 168

Patent

active

056437650

ABSTRACT:
A method for quantitative measurement of gene expression through multiplex competitive reverse transcriptase polymerase chain reaction amplification has been established which comprises isolating cellular mRNA of a target gene and a housekeeping gene which are then reverse transcribed and specifically amplified in the presence of competitive templates such that a ratio of target to housekeeping gene is obtained and used to assess the amount of gene expression. This method is useful for analysis of the small specimens (cells and tissues) available from human subjects and will allow improved understanding of mechanisms of disease.

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