Method for quantitative determination of polyamines

Details Method for quantitative determination of polyamines Method for quantitative determination of polyamines

1988-04-04

1992-01-21

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, 435 18, 435 19, 435189, 435190, 435183, 435859, C12Q 132, C12Q 126, C12N 904

Patent

active

050827707

ABSTRACT:
A method for the quantitative determination of polyamines, which comprises allowing a polyamine oxidizing enzyme, an .omega.-aminoalkylaldehyde dehydrogenase, an oxidized nicotinamide coenzyme and, as required, an acylpolyamine anidohydrolase to act upon a sample solution containing polyamines (for example, urine, blood and other kinds of body fluid), and measuring the reduced nicotinamide coenzyme thus formed by, for example, colorimetry, thereby determining the amount of said polyamines.

REFERENCES:
patent: 3929580 (1975-12-01), Forgione et al.
patent: 4444880 (1984-04-01), Tom
patent: 4460684 (1984-07-01), Bauer
patent: 4550078 (1985-10-01), Yamada et al.
Rosemblatt, M. S. et al., Biochemistry, vol. 13, No. 20, pp. 4176-4180 (1974).
Bachrach and Plesser, Anal. Biochem., 152, 423-431 (1986).
Russel & Russel, Clin. Chem., 21, No. 7, 860-863 (1975).

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