Method for quantitative determination of at least one chemical p

Details Method for quantitative determination of at least one chemical p Method for quantitative determination of at least one chemical p

1992-03-04

1993-08-03

Johnston, Jill A.

Chemistry: analytical and immunological testing

Metal or metal containing

Organometallic compound determined

436 79, 436 80, 436 81, 436144, 436172, G01N 2164, G01N 2129

Patent

active

052328589

ABSTRACT:
A method for the quantitative determination of one or more chemical parameters of a sample medium employs a fluorescent agent which is not responsive to the parameter to be determined and which has an excitation spectrum and an emission spectrum, and a closely adjacent chromogenic agent which responds to the parameter of the sample medium to be determined by a change in its absorption spectrum the emission spectrum of the fluorescent agent at least partically overlapping the absorption spectrum of the chromogenic agent. The absorption maximum of chromogenic agent is shifted in accordance with the parameter to be determined, causing a reduction of fluorescence decay time t in the fluorescent agent, thereby enabling quantitative determination of the chemical parameter with improved long-term stability and infrequent calibrations.

REFERENCES:
patent: 4367072 (1983-01-01), Vogtle et al.
patent: 4521511 (1985-01-01), Stout

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