Method for quantitation of antigens or antibodies on a solid sur

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Attached to antibody or antibody fragment or immunoglobulin;...

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23230B, 424 12, G01N 3300, G01N 3316, G21H 502

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active

040201511

ABSTRACT:
A method for quantitation of antigens or antibodies from a liquid sample on a solid surface. The sample is first sorbed directly to a nonimmunological surface. Then the surface is exposed to antibody or antigen labelled preferably with a fluorogen, to cause an immunological reaction to occur. After removal of unreacted reagent from the surface, the quantity of reacted labelled reagent is determined, as for example, fluorogen by a fluorometer. When the sample includes significant quantities of protein other than the antigen or antibody to be quantitated, buffer protein unreactive with the labelled antigen or antibody is added in concentrations sufficient to minimize the dependence of sorbed antigen or antibody upon variations in the concentration of such other protein.

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patent: 3646346 (1972-02-01), Catt
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Catt et al., J. of Lab. & Clin. Med., vol. 70, p. 820, 1967.
Wide, Acta Endocrinologia Supplementum, vol. 63, No. 142, pp. 207-221.

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