Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1995-02-22
1997-01-07
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 30, C12Q 102, C12Q 124
Patent
active
055915981
ABSTRACT:
A method is provided for the preparation of semisolid particles or cells that have been chemically derivatized resulting in hydrazide functionalities covalently incorporated onto cell surface membrane molecules of the semisolid particles or cells, as well as a method for determining the levels of hydrazide groups on the particles or cells. The method involves the preparation and analysis of red blood cells chemically derivatized in such a manner that hydrazide functionalities have been covalently incorporated onto the cell membrane molecules, the preparation of hydrazine derivatized cells and the use of the novel cells in agglutination reactions to promote the attachment of antigens and antibodies to the hydrazine derivatized cells without loss of antibody activity or the blocking of the epitope of interest.
REFERENCES:
patent: 4421896 (1983-12-01), Dorman
patent: 5329028 (1994-07-01), Ashkenazi
patent: 5349066 (1994-09-01), Kaneko
Muresan, V., A Hemagglutination Test for Binding of Hydrazide Derivatized Cytochemical Markers, Acta Histochem 79 193-98 (1986).
Traore, F., Determination of Picomole Amounts of Carbonyls as Luminarin Hydrazones by High Performance Liquid Chromatography with Fluorescence Detection, Chromatographia vol. 36 96-104 (1993).
In Vitro Attachment of Mono-and Oligosaccharides to Surface Clycoconjugates of Intact Cells, Tolvanen, M., et al. The Journal of Biological Chemistry vol. 261, No. 20 15 Jul. 1986 pp. 9546-9451.
Chow Herbert S.
Granados Edward N.
Heiman Daniel F.
Thaler Maria L.
Abbott Laboratories
Becker Cheryl L.
Gitomer Ralph
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