Method for quantitating and differentiating white blood cells

Details Method for quantitating and differentiating white blood cells Method for quantitating and differentiating white blood cells

1987-04-13

1989-11-21

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Biological cellular material tested

2504612, 356 39, 424 3, 436172, G01N 1510, G01N 2175, G01N 3350

Patent

active

048822841

ABSTRACT:
A method is provided for discriminating white blood cells from red blood cells and platelets in an unlysed, whole blood sample. The white blood cells may be discriminated into five subpopulations: lymphocytes, monocytes, neutrophils, eosinophils and basophils. A red light absorbing fluorescent dye is utilized along with a measurement system comprised of a red light source and suitable detectors for red fluorescence, forward light scatter and right angle scatter.

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