Method for quantifying cholesterol in high density lipoprotein

Details Method for quantifying cholesterol in high density lipoprotein Method for quantifying cholesterol in high density lipoprotein

2005-05-17

2005-05-17

Barts, Samuel (Department: 1623)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

C435S025000, C435S028000, C436S071000, C436S169000, C436S170000, C436S824000

Reexamination Certificate

active

06893832

ABSTRACT:
Disclosed is a method for quantifying cholesterol in HDL, which does not require complex fragmentation and separation operations, and by which the HDL cholesterol in test samples containing HDL and other lipoproteins such as low density lipoprotein (LDL), very low density lipoprotein (VLDL) and chylomicron (CM) may be quantified selectively, simply and accurately. The method for quantifying cholesterol in high density lipoprotein comprises a first step of erasing cholesterol in lipoproteins other than high density lipoprotein in a test sample, and a second step of adding a surfactant which specifically acts on high density lipoprotein to the product of the first step and enzymatically quantifying cholesterol in high density lipoprotein.

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