Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1995-06-07
1998-04-28
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
530397, 530412, 435 696, A61K 3824, A61K 3827, C04K 100, C04K 1400
Patent
active
057445877
ABSTRACT:
Methods of removing protein contaminants from a solution comprising thrombopoietin are disclosed. The solution is exposed to hydroxyapatite, whereby protein contaminants are bound to the hydroxyapatite and the thrombopoietin remains substantially unbound, and the unbound thrombopoietin is recovered. The use of hydroxyapatite is advantageously combined with other purification and concentration techniques, including ion-exchange chromatography, ligand affinity chromatography, hydrophobic interaction chromatography, ultrafiltration, and differential precipitation.
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Alaska Andrew R.
Chang Jin-Jyi
Downey William
Forstrom John W.
Phan Linh
Harle Jennifer
Parker Gary E.
Tsang Cecilia J.
ZymoGenetics Inc.
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