Method for purifying teicoplanin A 2

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Separation or purification

Reexamination Certificate

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C530S317000, C530S332000, C435S085000

Reexamination Certificate

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10621353

ABSTRACT:
Disclosed is a method for purifying teicoplanin A2comprising: (i) a primary pre-purification step of purifying a filtrate of fermentation broth of a strain using a synthetic adsorbent; (ii) a secondary pre-purification step of purifying the primary pre-purification solution using a cation exchange resin having a high cross-linkage, a catalytic resin or a chelate resin; (iii) a final purification step of purifying the secondary pre-purification solution using a reversed phase resin; and (iv) a powder-forming step. According to the present invention, it is possible to obtain teicoplanin A2with a higher purity through a relatively simple process without using an excessive amount of an organic solvent.

REFERENCES:
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patent: 479086 (1995-09-01), None
patent: 200066479 (2000-11-01), None
Parenti et al., “Teichomycins, New Antibiotics fromActinoplanes teichomyceticusNov. Sp.”, The Journal of Antibiotics, Apr. 1978, pp. 276-283.

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