Method for purifying somatotropin monomers

Details Method for purifying somatotropin monomers Method for purifying somatotropin monomers

1995-12-04

1998-06-30

Hutzell, Paula K.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530412, 530418, 530420, C07K 130

Patent

active

057735883

ABSTRACT:
There is provided a method for purifying a refold somatotropin monomer from a mixture of somatotropin monomers and somatotropin oligomers in an aqueous solution having a urea concentration of greater than 3 molar by the addition of an acid to reduce the pH of the solution to below about 7.7 to selectively precipitate the oligomers in the most part.

REFERENCES:
patent: 4612367 (1986-09-01), Grinnan et al.
patent: 5182369 (1993-01-01), Storrs et al.
H. Choi et al., Isolation and Purification of Human Growth Hormone in Yeast Cells Comprises Suspending Cells in Buffer Solution, Increasing pH, Removing Residue and Purification. Abstract, Derwent Publications Ltd., London, GB.

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