Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-05-31
2005-05-31
Carlson, Karen Cochrane (Department: 1653)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C530S417000, C530S419000
Reexamination Certificate
active
06900034
ABSTRACT:
The invention relates to a method for solubilising and purifying recombinant proteins which are expressed in bacterial host cells and are deposited as insoluble aggregates (inclusion bodies). The purification is based on conversion of the inclusion bodies into soluble forms using organic denaturing reagents and using chromatographic methods. Inorganic, alkaline, salt-containing eluents are selected here which, after purification is complete, enable the recombinant proteins, after neutralisation, to be made available in a form which can be employed directly for medical use and is physiologically acceptable. The method is particularly suitable for purifying allergens and allergen fragments.
REFERENCES:
patent: 2002/0018779 (2002-02-01), Valenta et al.
patent: 0432419 (1991-06-01), None
patent: WO 9814467 (1998-04-01), None
Ferreira, F. et al. “Purification and Characterization of Recombinant Bel v 1, the Major Birch Pollen Allergen.” Journal of Biological Chemistry, vol. 268, No. 26, pp. 19574-19580 (1993).*
Fischer, B. “Renaturation of Recombinant Proteins Produced As Inclusion Bodies.” Biotech Adv. vol. 12, pp. 89-101 (1994).*
Lorenz A R et al., “Recombinant food allergens,” Journal of Chromatography, May 25, 2001, pp. 255-279, Amsterdam.
Cromwell Oliver
Fiebig Helmug
Suck Roland
Carlson Karen Cochrane
Desai Anand
Merck Patent GmbH
Millen White Zelano & Branigan P.C.
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