Method for purifying polypeptides

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Separation or purification

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530399, 530413, 530300, 530350, 530351, 530324, 514 21, A61K 3818, A61K 3830, C07K 122, C07K 14475

Patent

active

054516609

ABSTRACT:
A process is provided for selectively separating a polypeptide of interest from components of differing hydrophobicity in a mixture comprising the steps of:

REFERENCES:
patent: 3869482 (1975-03-01), Wolfe
patent: 3876775 (1975-04-01), Izaka et al.
patent: 3904751 (1975-09-01), Zwisler et al.
patent: 4199450 (1980-04-01), Dulout et al.
patent: 4652529 (1987-03-01), Collins et al.
patent: 4677192 (1987-06-01), Obermeier et al.
patent: 4725673 (1988-02-01), Herring
patent: 4732683 (1988-03-01), Georgiades et al.
patent: 4738926 (1988-04-01), Hamada et al.
patent: 4777242 (1988-10-01), Nelles
patent: 4849434 (1989-07-01), Enomoto et al.
patent: 4894330 (1990-01-01), Hershenson et al.
patent: 4902783 (1990-02-01), Goda et al.
patent: 4908432 (1990-03-01), Yip
patent: 5004688 (1991-04-01), Craig et al.
patent: 5028531 (1991-07-01), Ueder et al.
patent: 5057426 (1991-10-01), Henco et al.
patent: 5071959 (1991-12-01), Capron et al.
patent: 5084384 (1992-01-01), Wong et al.
patent: 5158875 (1992-10-01), Miller et al.
patent: 5231178 (1993-07-01), Holtz et al.
patent: 5278284 (1994-01-01), Lusk et al.
Horvath--High Performance Liquid Chromatography vol. I 76-80 1980.
Horvath--High Performance Liquid Chromatography vol. 2, 124-127 & 131-134 1980.
Heftman "Chromatography" Part A A139-A140 & A166-A167 1983.
Stock et al. "Chromatographic Methods" 26-32 & 37-38 1974.
Adachi et al., "Effects of Differences in Charge and Hydrophobicity of Surface Amino Acids of Hemoglobins on High-Performance Gel-Permeation Chromatography", J. Chromatogr., 428:247-254 (1988).
Anspach et al., "Comparative Study of Zorbax Bio Series FG 250 and GF 450 and TSK-Gel 3000 SW and SWXL Columns in Size-Exclusion Chromatography of Proteins", J. Chromatog., 443:45-54 (1988).
Bock et al., "Protein Purification: Adsorption Chromatography on Controlled Pore Glass with the Use of Chaotropic Buffers", Science, 191:380-383 (1976).
Byrne et al., "Procedure for isolation of Gangliosides in High Yield and Purity: Simultaneous Isolation of Neutral Glycosphingolipids", Analytical Biochem., 148:163-173 (1985).
Chadha et al., "Chromatography of Human Leukocyte Interferon on Controlled Pore Glass", Prep. Biochem., 11:467-482 (1981).
Chadha et al., "Adsorption of Human Alpha (Leukocyte) Interferon on Glass: Contributions of Electrostatic and Hydrophobic Forces", J. Interferon Re., 2:229-234 (1982).
Cornell et al., "Isolation of Insulin-Like Growth Factors I and II from Human Plasma", Prep. Biochem., 14:123-138 (1984).
Duhamel et al., "Isomerisation Oxaziridine-Amide Sur Gel De Silice Obtention Non Classique D'Une Liaison Peptidique", Tetrahedron Lett., 26:6065-6066 (1985).
Edy et al., "Purifcation of Interferon by Adsorption Chromatography on Controlled Pore Glass", J. Gen. Virol., 33:517-521 (1976).
Flouret et al., "Synthesis of Oxytocin Using Iodine for Oxidative Cyclization and Silica Gel Adsorption Chromatography for Purification", Int. J. Pept. Protein Res., 13:137-141 (1979).
Hemmasi et al., "Synthesis of the C-terminal Undeca-and Protected Docosapeptide of Bovine Insulin B-Chain", Hoppe-Seyler's Z. Physiol Chem., 365:485-492 (1984).
Hillar et al., "Small Peptides Bound to Polysomal RNA Inhibit Gene Expression in Cell-Free Systems, Replication of Stimulated Lymphocytes and DNA Repair in Isolated Chromatin", Physiol. Chem. Phys. Med. NMR, 17:307-323 (1985).
Hillar et al., "Nuclear Peptides from Calf Liver: Large Scale Isolation and Fractionation; Control of Gene Expression in Cell-Free Systems, and Inhibition of Growth of Cells in Culture" Physiol. Chem. Phys. Med. NMR, 17:325-343 (1985).
Hillar et al., "Nuclear Peptides from Calf Liver: Large Scale Isolation and Fractionation: Control of Gene Expression in Cell-Free-Systems, and Inhibition of Growth of Cells in Culture", Bas. Appl. Histochem., 31:299-313 (1987).
Huennekens et al., "Transport of Folate Compounds into Mammalian and Bacterial Cells", Chemistry and Biology of Pteridines, ed. by Pfleidever (Berlin, de Gruyter, 1975), pp. 179-196.
Jentsch et al., "Bee Venom Peptides XVIII Peptide-M and MCD-Peptide: Isolation and Characterization", Int. J. Pept. Protein Res., 9:78-79 (1977).
Kiselev et al., "Molecular Sieve Chromatography of Polymers and Proteins on Macroporous Silica Gels", in Column Chromatography, 5th Int. Symposium (Lausanne 7-10 Oct. 1969) pp. 124-125.
Loginova et al., Priki, Biokhim, Mikrobiol., 14:715-718 (1978) (Abstract in English).
Lu et al., Shengwu Huazue Yu Shengwu Wuli Jinzhan 48:4648 (1982).
Manning et al., "Reversed-Phase Liquid Chromatography of Elastin Peptides", J. Chromatog., 487:41-50 (1989).
Mizutani, "Adsorption Chromatography of Biopolymers on Porous Glass", J. Lig. Chromatog., 8:925-983 (1985).
Mizutani, "Decreased Activity of Proteins Adsorbed onto Glass Surfaces with Porous Glass as a Reference":, J. Pharm. Sci., 69:279-280 (1980).
Mizutani, "Adsorption of Antibody and Globulin onto Glass Surfaces", J. Pharm. Sci., 69:1226-1227 (1980).
Mizutani et al., "Comparison of Elution Patterns of Proteins Chromatographed on Controlled-Pore Glass and CarboxymethylCellulose", J. Chrom. 168:143-150 (1979).
Moks et al., "Large-Scale Affinity Purification of Human Insulin-Like Growth Factor I from Culture Medium of Escherichia Coli" Bio/Technology, 5:379-382 (1987).
Mordarski et al., "Antibiotics Produced By Streptomyces Olivaceus 142.II. Isolation, Purification and Activity Spectrum of Antibiotic WR 142-FPG", Archivum Immunologiae et Therapiae Experimentalis, 25:273-283, (1977).
Morise et al., "Biological Activity of Partially Purified Digitalis-like Substance and Na-K-ATPase Inhibitor in Rats", Japanese Circulation Journal, 52:1309-1316 (1988).
Mount et al., "Purification and Characterization of Epidermal Growth Factor (.beta.-Urogastrone) and Epidermal Growth Factor Fragments from Large Volumes of Human Urine", Archives of Biochem. and Biophys., 240(1):32-42, (1985).
Pan et al., "Structural Characterization of Human Interferon .UPSILON.", Eur. J. Biochem., 166:145-149, (1987).
Pickart et al., "Purification of Growth-Promoting Peptides and Proteins, and of Histones, By High Pressure Silica Gel Chromatography", Preparative Biochemistry, 5(5 & 6):397-412, (1975).
Pillot et al., "Immunochemical Structure of the Hepatitis B Surface Antigen Vaccine-I. Treatment of Immobilized HBsAg By Dissociation Agents with or without Enzymatic Digestion and Identification of Polypeptides By Protein Blotting", Molec. Immun. 21(1):53-60, (1984).
Schall et al., "Human Macrophage Inflammatory Protein .sigma. (MIP-1.sigma.) and MIP-1.beta. Chemokines Attract Distinct Populations of Lymphocytes", J. Exp. Med., 177:1821-1825, (1993).
Schmidt et al., "High Performance Liquid Chromatography of Proteins on a Diol-Bonded Silica Gel Stationary Phase", Anal. Chem., 52:177-182, (1980).
Singhalj et al., "Presence of Fucolipid Antigens with Mono-and Dimeric X Determinant (Le.sup.x) in the Circulating Immune Complexes of Patients with Adenocarcinoma", Cancer Res., 47:5566-5571, (1987).
Skogen et al., "Endogenous Digoxin-like Immunocreative Factors Eliminated from Serum Samples by Hydrophobic Silica-Gel Extraction and Enzyme Immunoassay", Clin. Chem., 33/3:401-404, (1987).
Sofer, "Current Applications of Chromatography in Biotechnology", Bio/Tech., 4:712-715, (1986).
Stankovic et al., "Purification of Gramicidin A", Anal., Biochem., 184:100-103, (1990).
Stoffel et al., "The Primary Structure of Bovine Brain Myelin Lipophilin (Proteolipid Apoprotein)", Hoppe-Seyler's Z. Physiol Chem., 364:1455-1466, (1983).
Sulkowski, "Purification of Porteins by IMAC", Trends in Biotechnology, 3:1-7 (1985).
Takagi et al., "Effect of Salt Concentration on the Elution Properties of Complexes Formed Between Sodium Dodecylsulphate and Protein Polypeptides in High-Performance Silica Gel Chromatography", J. of Chroma., 208:201-208, (1981).
Tao et al., "Isolation and Characterization of Human Urinary Colony-Stimulating Factor", Bio. Chem. Hoppe-Seyler, 368:187-194, (1987).
Trucksess et al., "Extraction, Cleanup, and Quantitative Determination of Aflatoxins B.sub.1 and M.sub.1 in Beef Liver", J. Assoc. Off. Anal. Che

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for purifying polypeptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for purifying polypeptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for purifying polypeptides will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1829131

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.