Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Separation or purification
Patent
1990-08-28
1991-08-27
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Separation or purification
530331, C07K 114, C07K 508
Patent
active
050434233
ABSTRACT:
This invention firstly provides a method for purifying particular compounds of peptide or pseudo-peptide structure in which the number of protonable basic functions is greater than the number of acid functions and which have a molecular weight of less than 1000 daltons, by ion exchange displacement chromatography. In the method of the present invention the stationary phase used is a cationic exchange resin or a cross-linked polymer matrix activated with acid groups; the transporter solvent used is water if the compound to be purified already possesses at least one net positive charge, or aqueous dilute solutions of inorganic or strong organic acids which protonate the basic groups of the peptide or pseudo-peptide to be separated without modifying the structure of the peptide compound, such as acetic acid, trifluoroacetic acid, formic acid, hydrochloric acid or sulphuric acid; the displacer compound used is a triethylenetetraammonium salt.
REFERENCES:
patent: 3778426 (1973-12-01), Najjar
patent: 4855405 (1989-08-01), Yoshioka et al.
Horvath et al., "Operating Parameters in High-Performance Discplacement Chromatography," J. Chromatogr. 255, 273-93 (1983).
Cardinali Franco
Longobardi Maria G.
Viscomi Giuseppe C.
Russel Jeffrey E.
Sclavo S.p.A.
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