Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1995-05-31
1997-06-24
Arthur, Lisa B.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530412, 530415, 530416, 530417, 530418, C07K 114, C07K 118, C07K 120, C07K 122
Patent
active
056418698
ABSTRACT:
A novel polypeptide with binding affinity for the p185.sup.HER2 receptor, designated heregulin-.alpha., has been identified and purified from cultured human cells. DNA sequences encoding additional heregulin polypeptides, designated heregulin-.alpha., heregulin-.beta.1, heregulin-.beta.2, heregulin-.beta.2-like, and heregulin-.beta.3, have been isolated, sequenced and expressed. Provided herein are nucleic acid sequences encoding the amino acid sequences of heregulins useful in the production of heregulins by recombinant means. Further provided are the amino acid sequences of heregulins and purification methods therefor. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4882275 (1989-11-01), Klagsburn
patent: 4968603 (1990-11-01), Slamon et al.
patent: 5169837 (1992-12-01), Lagarde et al.
patent: 5237056 (1993-08-01), Fischbach
"Using the PolyCAT A col." PolyLC: HPLC Supplies for the Life Sciences (and Application Note: HPLC of Hemoglobins on PolyCAT A), Columbia, MD:PolyLC Inc.
Alpert, "Cation-exchange high-performance liquid chromatography of proteins on poly(aspartic acid)-silica" J. Chromatogr. 266:23-37 (1983).
Benveniste et al., "Purification and characterization of a human T-lymphocyte-derived glial growth-promoting factor" Proc. Natl. Acad. Sci. 82:3930-3934 (1985).
Brockes, "Assay and isolation of glial growth factor from the bovine pituitary" Methods in Enzymology 147:217-225 (1987).
Brockes et al., "Glial growth factor-like activity in Schwann Cell tumors" Annals of Neurology 20:317-322 (1986).
Brockes et al., "Purification and preliminary characterization of a glial growth factor from the bovine pituitary" Journal of Biological Chemistry 255(18):8374-8377 (1980).
Davis et al., "Isolation and characterization of a neu protein-specific activating factor from human ATL-2 cell conditioned medium" Biochem. & Biophys. Res. Comm. 179(3):1536-1542 (1991).
Davis et al., "Platelet-derived growth factors and fibroblast growth factors are mitogens for rat Schwann Cells" Journal of Cell Biology 110:1353-1360 (1990).
De Corte et al., "A 50 kDa protein present in conditioned medium of COLO-16 cells stimulates cell spreading and motility, and activates tyrosine phosphorylation of Neu/HER-2, in human SK-BR-3 mammary cancer cells" J. Cell Science 107:405-416 (1994).
Dobashi et al., "Characterization of a neu/c-erbB-2 protein-specific activating factor" Proc. Natl. Acad. Sci. 88:8582-8596 (1991).
Falls et al., "ARIA, a protein that stimulates acetylcholine receptor synthesis, is a member of the Neu ligand family" Cell 72:801-815 (1993).
Fitzgerald et al., "Characterization and Sequence Analysis of the Human Ornithine Decarboxylase Gene" DNA 8(9):623-634 (1989).
Gray et al., "Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor" Nature 303:722-725 (1983).
Hoffman, Michelle, "New Clue Found to Oncogene's Role in Breast Cancer" Science 256:1129 (1992).
Holmes et al., "Identification of heregulin, a specific activator of p185.sup.erbB2 " Science 256:1205-1210 (1992).
Hudziak et al., "p185.sup.HER2 Monoclonal Antibody Has Antiproliferative Effects In Vitro and Sensitizes Human Breast Tumor Cells to Tumor Necrosis Factor" Molecular & Cellular Biology 9(3):1165-1172 (1989).
Kimura et al., "Structure, expression and function of a schwannoma-derived growth factor" Nature 348:257-260 (1990).
Kokai et al., "Phosphorylation process induced by epidermal growth factor alters the oncogenic and cellular neu (NGL) gene products" Proc. Natl. Acad. Sci USA 85:5389-5393 (1988).
Kunisada et al., "Sequence Organization of Repetitive Sequences Enriched in Small Polydisperse Circular DNAs from HeLa Cells" J. Mol. Biol. 198:557-565 (1987).
Lemke & Brockes, "Identification and purification of glial growth factor" J. Neurosci. 4(1):75-83 (1984).
Lupu et al., "Direct interaction of a ligand for the erbB2 oncogene product with the EGF receptor and p185.sup.erbB2 " Science 249:1552-1555.
Lupu et al., "Purification and Characterization of a Novel Growth Factor from Human Breast Cancer Cells" Biochemistry 31:7330-7340 (1992).
Lupu et al., "Purification of a novel growth factor that binds exclusively to the erbB-2 receptor protein and induces cellular responses" Proc. Am. Assoc. Cancer Res. 32:abst. No. 297, p. 50 (1991).
Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harber Lab. pp. 412-433 (1982).
Marchionni et al., "Glial growth factors are alternatively spliced erbB2 ligands expressed in the nervous system" Nature 362:312-318 (1993).
Nagata et al., "Solution structure of the epidermal growth factor-like domain of heregulin-.alpha., a ligand for p180.sup.erbB-4 " EMBO J, 13(15):3517-3523.
Peles et al., "Isolation of the Neu/HER-2 Stimulatory Ligand: A 44 Kd Glycoprotein That Induces Differentiation of Mammary Tumor Cells" Cell 69(1):205-216 (1992).
Pohlenz et al., "The Human VK Locus, Characterization of Extended Immunoglobulin Gene Regions by Cosmid Cloning" J. Mol. Biol. 193:241-253 (1987).
Sliwkowski et al., "Coexpression of erbB2 and erbB3 proteins reconstitutes a high affinity receptor for heregulin" Journal of Biological Chemistry 269(20):14661-14665 (1994).
Smith et al., "The Major Constituents of Cells" Principles of Biochemistry: General Aspects, McGraw-Hill Book Co., New York p. 164 (7th Edition 1983).
Tarakhovsky et al., "A 25 kDa polypeptide is the ligand for p185Neu and is secreted by activated macrophages" Oncogene 6(12):2187-2196 (1991).
Wen et al., "Neu differentiation factor: a transmembrane glycoprotein containing an EGF Domain and an Immunoglobulin Homology Unit" Cell 69(3):559-572 (1992).
Xu et al. Eighty-Second Annual Meeting of the American Association for Cancer Research Proceedings 32:260 (Abstract No. 1544 1991).
Yarden, "Biochemical Analysis of the Ligand for the neu Oncogenic Receptor" Biochemistry 30:3543-3550 (1991).
Yarden et al., "Experimental approaches to hypothetical hormones: detection of a candidate ligand of the neu protooncogene" Proc. Natl. Acad. Sci. USA 86:3179-3183 (1989).
Holmes William E.
Vandlen Richard L.
Arthur Lisa B.
Genentech Inc.
Lee Wendy M.
LandOfFree
Method for purifying heregulin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for purifying heregulin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for purifying heregulin will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-150030