Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1996-05-09
1998-12-15
Kemmerer, Elizabeth C.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530416, 530417, 530351, 435 695, C07K 114, C07K 118, C07K 136, C07K 14535
Patent
active
058498834
ABSTRACT:
Processes for isolating and purifying granulocyte colony stimulating factor (G-CSF) from a G-CSF producing microorganism are disclosed. The simplified processes include steps of lysing the microorganism and separating insoluble material containing G-CSF from soluble proteinaceous material; extracting the material with deoxycholate (optionally); solubilizing and oxidizing the G-CSF in the presence of a denaturant solubilizing agent and an oxidizing agent; removing the denaturant solubilizing agent from the G-CSF; subjecting the G-CSF to ion exchange chromatography; and recovering the purified G-CSF; yet excludes other cumbersome purification steps.
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Andresen Jeffrey W.
Boone Thomas C.
Miller Allan L.
Amgen Inc.
Crandall Craig A
Kemmerer Elizabeth C.
Levy Ron K
Odre Steven M
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