Method for purifying gamma-interferon

Details Method for purifying gamma-interferon Method for purifying gamma-interferon

1984-02-07

1985-02-12

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

424 85, 435 68, 435272, 435811, 436548, A61K 4502, C07G 700

Patent

active

044990146

ABSTRACT:
A method is provided for restoring some or all of the activity of gamma-interferon which has been in contact with an acidic solution comprising the steps of: (a) placing the gamma-interferon in a solution which has a pH between about 5.5 and 9.5; and (b) incubating the solution at a temperature of between about 2.degree. C. and 8.degree. C. for a period of at least 24 hours. The method allows antibody affinity chromatography employing acid elution to be used to purify gamma-interferon, in that, the activity of the acid-eluted gamma-interferon can be essentially completely restored using the reactivation process.

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