Method for purifying galactosyltransferase isoenzymes and produc

Details Method for purifying galactosyltransferase isoenzymes and produc Method for purifying galactosyltransferase isoenzymes and produc

1979-03-05

1980-05-27

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435815, 424 94, C07G 7026

Patent

active

042051290

ABSTRACT:
Pure galactosyltransferase isoenzyme GT-II having a molecular weight of about 71,000 is obtained from malignant cells or tumors of animals and humans. The GT-II is isolated by subjecting a body fluid or extract of the malignant cells or tumors containing the isoenzyme and GT-I galactosyltransferase isoenzyme to serial column separation steps and utilizing materials in the column which resemble cofactors for galactosyltransferase GT-II.

REFERENCES:
Podolsky et al., Biochemical and Biophysical Research Communication, vol. 65, No. 2, pp. 545-551, Jul. 1975.

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