Method for purifying fibroblast growth factor protein

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

514 2, 514 54, 514 57, 514802, 530399, 530350, 530413, 530383, C12N 1500, C07K 320, C07K 1300, C12P 2102

Patent

active

051207150

ABSTRACT:
Disclosed is a method for purifying a fibroblast growth factor (FGF) protein with use of a crosslinked polysaccharide sulfate. The FGF protein is preferable a mutein, in which at least one human basic FGF-constituent amino acid is substituted by at least one different amino acid. The crosslinked polysaccharide sulfate is preferably a crosslinked cellulose sulfate, a crosslinked agarose sulfate or a crosslinked dextran sulfate. According to the present invention, FGF can be highly purified on a commercial scale, and therefore preparations containing the FGF protein can be advantageously formulated.

REFERENCES:
patent: 4083961 (1978-04-01), Dussourd'Hinterland et al.
patent: 4785079 (1988-11-01), Gospodarowicz et al.
patent: 4789733 (1988-12-01), Winkelman
patent: 4798886 (1989-01-01), Kato et al.
patent: 4840941 (1989-06-01), Ueno et al.
patent: 4999340 (1991-03-01), Hoffman et al.
patent: 5002934 (1991-03-01), Norton et al.
patent: 5006642 (1991-04-01), Newman et al.
Huang, et al., The Journal of Biological Chemistry, 261(25):11600-11607 (1986).
Research Disclosure, Feb. 1989 "Application of Sulfated Polysaccharide Gel for Affinity Chromatography."
A pamphlet of Sulfated Cellulofine (Seikagaku Kogyo Co.).
J. Biol. Chem. 261, 1924-1928 (1986), R. Lobb, et al.
M. Senoo, et al., Biochemical and Biophysical Research Communications, 151, 701-708 (1988).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for purifying fibroblast growth factor protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for purifying fibroblast growth factor protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for purifying fibroblast growth factor protein will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1804500

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.