Method for purifying factor VIII and preparations obtained

Details Method for purifying factor VIII and preparations obtained Method for purifying factor VIII and preparations obtained

1992-09-23

1994-12-06

Schain, Howard E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530381, 530416, 530417, 530413, 530384, 530420, 530427, A61K 3516, C07G 700, C07K 1300, C07K 3120

Patent

active

053711955

ABSTRACT:
In the method for purifying factor VIII from cryoprecipitate, which is dissolved and then treated with alumina gel, the extract is diluted to a protein concentration not exceeding approximately 5 g/l and subjected to viral inactivation with solvent/detergent, the inactivated extract containing the solvent/detergent is then subjected to chromatography on a weak anion exchange column which is hydrophilic in nature and factor VIII is then eluted with a dissociating buffer.

REFERENCES:
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patent: 4508709 (1985-04-01), Amphlett et al.
patent: 4743680 (1988-05-01), Mathews et al.
patent: 4758657 (1988-07-01), Farb et al.
patent: 5043428 (1991-08-01), Heimburger et al.
patent: 5110907 (1992-05-01), Kosow et al.
Vehar et al; "Preparation and Properties of Bovine . . . "; Biochem, vol. 19, No. 3, Feb. 5, 1980, pp. 401-410.
Faure et al; "Improved Buffer for the Chromatographic . . . "; Journ of Chromatography, vol. 257, 1981, pp. 387-391.
Lundblad et al; "the Effect of Dextrose on Chromatography . . . "; Thrombosis Research, vol. 1, 1972, pp. 197-200.

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