Method for purifying enzymes from clostridium histolyticum...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S183000, C424S094670, C424S094650, C424S094630, C424S094600, C210S634000, C210S656000, C210S667000

Reexamination Certificate

active

07083964

ABSTRACT:
The invention relates to a method for purifying at least one enzyme obtained in an excess fermentation ofClostridium histolyticum.It is provided for that the enzymes of the excess fermentation are separated by a multistage chromatography method by exclusively using chromatography materials on styrene/divinyl-benzene base and/or on base of in particular ceramic hydroxylapatite.

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