Method for purifying cholera toxin

Details Method for purifying cholera toxin Method for purifying cholera toxin

1998-03-06

1999-12-28

Saucier, Sandra E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530825, C07K 1714, C07K 1428

Patent

active

060083298

ABSTRACT:
The invention relates to a method of purifying cholera toxin using a matrix with at least one ion chosen from among matrix with Ni.sup.+2, Co.sup.+2, Cd.sup.2 or Zn.sup.+2 immobilized thereon. It is possible thereby to selectively elute the B subunit for cholera toxin from the matrix.

REFERENCES:
patent: 5563046 (1996-10-01), Mascarenhas et al.
patent: 5674677 (1997-10-01), Peterson
Buckley et al., "The role of calcium influx in cellular proliferation indd by interaction of endogenous ganglioside GM1 with the B subunit of cholera toxin", Biochimica et Biophusica Acta, 1995, 1256, pp. 275-283.
Dertzbaugh et al., "Cholera toxin B-subunit gene fusion: structural and functional analysis of the chimeric protein", Infection and Immunity, 1990, vol. 58, No. 1, pp. 70-79.
Kazemi et al., "Mapping epitopic regions of cholera toxin B-subunit protein", Molecular Immunology, 1991, vol. 28, No. 8, pp. 865-876.
Clements et al., "Immunological cross-reactivity between a heat-labile enterotoxin(s) of E.coliand subunits of Vibrio cholerae enterotoxin", Infection and IMmunity, 1978, vol. 21, No. 3, pp. 1036-1039.

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