Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2004-07-08
2009-10-06
Allen, Marianne P (Department: 1647)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S300000, C530S350000, C530S351000, C530S380000, C530S381000, C530S399000, C530S412000
Reexamination Certificate
active
07598356
ABSTRACT:
The present invention relates to a two-step method for isolating proteins from the cystine-knot superfamily based on dye ligand affinity chromatography and reversed-phase chromatography. Advantageously, the method can be performed in a relatively short period of time, involves inexpensive reagents, and requires little sample preparation before and during the purification process. Protein fusions between cystine-knot proteins and proteins of interest are further provided for the isolation of said protein of interest or complexes containing said protein of interest using the two-step method disclosed.
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Bedows Elliott
Wilken Jason A.
Allen Marianne P
Board of Regents of the University of Nebraska by and on Behalf
Senniger Powers LLP
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