Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1987-06-15
1990-02-20
Moskowitz, Margaret
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530412, 530427, 435 691, C07K 318, C07K 312, C07K 328
Patent
active
049027832
ABSTRACT:
There is disclosed a method for purifying a gene-expression product produced by recombinant DNA technique which comprises a specific sequence of steps including adsorption treatment with silica gel, adsorption treatment with activated carbon, at least twice density gradient centrifugation and at least twice equilibrium density gradient centrifugation. The method of the present invention is very effective to remove allergen from gene-expression products contaminated therewith, enabling highly purified gene-expression products to be produced on a large scale.
REFERENCES:
patent: 3636191 (1972-01-01), Blumberg
patent: 3951937 (1976-04-01), Vneck
patent: 4503035 (1985-03-01), Pestka et al.
patent: 4505893 (1985-03-01), Mori et al.
patent: 4508709 (1985-04-01), Amphlett et al.
patent: 4508833 (1985-04-01), Sonneborn et al.
patent: 4514506 (1985-04-01), Braatz et al.
patent: 4525459 (1985-06-01), Fletcher
patent: 4552758 (1986-11-01), Murphy et al.
patent: 4554157 (1985-11-01), Skelly et al.
patent: 4565697 (1986-01-01), Ohmura et al.
patent: 4578269 (1986-03-01), Morein
patent: 4649192 (1987-03-01), VanWijnendoele et al.
Hassler, John W., Purification with Activated Carbon, N.Y., N.Y., Chemical ublishing Co., Inc., 1974, pp. 102-106.
Osterman, L. A., Methods of Protein and Nucleic Acid Research, vol. 1, N.Y., Springer--Verlag, 1984, pp. 241, 248, 249, 284, 285.
Methods in Microbiology, vol. 5B, 425-454, (1971), Academic Press, England.
Proc. Natl. Acad. Sci., 76, 5601-5605, (1979).
Nucleic Acids Research, 11, 2745-2763, (1983).
Akiyama Toshiyuki
Goda Hideo
Konobe Takeo
Takaku Keisuke
Takamizawa Akihisa
Furman Keith C.
Moskowitz Margaret
The Research Foundation for Microbial Diseases of Osaka Universi
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