Method for purification of uricase

Details Method for purification of uricase Method for purification of uricase

1982-03-01

1983-07-19

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 10, 435814, C12N 906, C12Q 162

Patent

active

043944507

ABSTRACT:
A method is provided for purifying uricase by decreasing the amount of active catalase present. The method involves adjusting the pH of a catalase-containing uricase preparation to a pH in the range of about 11 to 13 to inactivate the catalase and recovering a uricase preparation substantially free of active catalase.

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patent: 4064010 (1977-12-01), Harris
Watanabe et al, Analytical Biochemistry, vol. 89, pp. 343-347, (1978).
J. Biol, Chem., 216:625-641, 1955. "Studies on Uricase", by H. R. Mahler, G. Hubscher and H. Baum.

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