Method for purification of recombinant proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Separation or purification

Reexamination Certificate

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C530S412000, C530S413000, C549S263000, C549S299000, C549S300000, C549S200000

Reexamination Certificate

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09839696

ABSTRACT:
Purification of poly-amino acid-tagged recombinant proteins has been improved by the use of a carboxymethylated aspartate ligand complexed with a third-block transition metal having an oxidation state of 2+and a coordination number of 6. A method for synthesizing the metal ion-CM-Asp complex is also described. Further, the metal ion-CM-Asp complex can be used for screening protein function.

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