Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Separation or purification
Patent
1996-08-16
1999-10-05
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Separation or purification
530412, 530413, 549263, 549299, 549300, C07K 122
Patent
active
059626410
ABSTRACT:
Purification of poly-amino acid-tagged recombinant proteins has been improved by the use of a carboxymethylated aspartate ligand complexed with a third-block transition metal having an oxidation state of 2.sup.+ and a coordination number of 6. A method for synthesizing the metal ion-CM-Asp complex is also described. Further, the metal ion-CM-Asp complex can be used for screening protein function.
REFERENCES:
patent: 5047513 (1991-09-01), Dobeli et al.
Mantovarra et al. (1991) "Further Characterization of Carboxymethylated Aspartic Acid Agarose. Purification of Human .alpha.2-Macroglubulin and Hemopexin" Biotech. & Applied Biochem. 13: 371-9.
Crowe et al. (1995) "One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin" Mol. Biotech. 4:247-58.
Mantovaara et al. `Further Characterization of Carboxymethylated Aspartic Acid Agarose of Human .Alpha.(2)-Macroglobulin and Hemopexin`, Biotechnolgoy and Applied Biochemistry, vol. 13, pp. 371-379, 1991.
Mantovaara et a., `Carboxymethylated Aspartic Acid Agarose, A Selective Adsorbent for Calcium Binding Proteins, Preliminary Studies`, Biotechnology and Applied Biochemistry, vol. 13. pp. 315-322, 1991.
Presland et al., `Characterization of Two Distinct Calcium Binding Sites in the Amino Terminus of Human Profilaggrin`, Journal of Investigative Dermatology, vol. 4, No. 2, pp. 218-223, 1995.
Porath, J. et al. (1975) "Metal chelate affinity chromatography, a new approach to protein fractionation" Nature 258:598-599.
Porath, J., B. Olin (1983) "Immobilized Metal Ion Affinity Adsorption and Immobilized Metal Ion Affinity Chromatography of Biomaterials. Serum Protein Affinities for Gel-Immobilized Iron and Nickel Ions" Biochemistry 22:1621-1630.
Hochuli, E. et al. (1987) "New Metal Chelate Adsorbent Selective for Proteins and Peptides Containing Neighbouring Histidine Residues" Journal of Chromatography 411:177-184.
Mantovaara, T. et al. (1989) "Purification of Human Serum Amyloid P Component (SAP) by Calcium Affinity Chromatography" Biotehcnology and Applied Biochemistry 11:564-570.
Mantovaara, T. et al. (1991) "Carboxymethylated Aspartic Acid Agarose, a Selective Adsorbent for Calcium-Binding Proteins, Preliminary Studies" Biotehcnology and Applied Biochemistry 13:315-322.
Mantovaara, T. et al. (1991) Purification of Factor VIII:c Coagulant Activity from Rat Liver Nonparenchymalk Cell Culture Medium by Immobilized Metal Ion Affinity Chromatography Biotechnology and Applied Biochemistry 13: 120-126.
Ford, C.F. et al. (1991) "Fusion Tails for the Recovery and Purification of Recombinant Proteins" Protein Expression and Purification 2:95-107.
Porath, J. (1992) "Immobilized Metal Ion Affinity Chromatography" Protein Expression and Purification 3:263-281.
Kain Steven R.
Nelson Paul S.
Yang Te-Tuan
Adler Benjamin Aaron
Clontech Laboratories, Inc.
Gupta Anish
Tsang Cecilia J.
LandOfFree
Method for purification of recombinant proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for purification of recombinant proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for purification of recombinant proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1172954