Method for purification of recombinant copper/zinc (CU-ZN) super

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435815, 435816, C12N 902

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active

053607295

ABSTRACT:
The subject invention provides a method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypeptide analog thereof having substantially the same amino acid sequence as, and the bioloical activity of, naturally-occurring Cu-Zn superoxide dismutase from a composition which comprises cells containing Cu-Zn superoxide dismutase or a polypeptide analog thereof. The invention also provides a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog.

REFERENCES:
patent: 4742004 (1988-05-01), Hartman et al.
Weng, Q. et al. (1989) Chem. Abst. 110:53519v.
Grunow, M., et al. (1988) Chem. Abst. 109:225811j.
Fridovich, I. (1986) Enzymology 58, 61-97.
Ksjihara, J. I., et al. (1988) J. Biochem 104, 638-642.

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