Method for purification of nucleic acids by phase separation...

Details Method for purification of nucleic acids by phase separation... Method for purification of nucleic acids by phase separation...

2008-09-30

2008-09-30

Sisson, Bradley L (Department: 1634)

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

C435S270000, C436S094000, C536S023100

Reexamination Certificate

active

11265461

ABSTRACT:
An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.

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