Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification
Patent
1985-03-08
1988-04-19
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Recovery or purification
435 5, 435 68, 435174, 435176, 435235, 435803, 435820, 436161, 436543, 530417, 530826, C12N 702
Patent
active
047389265
ABSTRACT:
Industrially useful method for the purification of HBs antigen produced by a recombinant organism being capable of producing HBs antigen which is prepared by means of DNA recombination technique, which comprises subjecting an HBs antigen-containing material produced by a recombinant organism to an adsorption chromatography with a silica, optionally followed by a gel filtration and further an adsorption chromatography with a hydroxyapatite, and then eluting the HBs antigen, preferably, with a buffer having a pH 9 or more which is incorporated with urea. The purification method can give a highly pure HBs antigen suitable for the preparation of hepatitis B vaccine in a large scale from recombinant organisms prepared by DNA recombination technique.
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Adachi Satoshi
Hamada Fukusaburo
Mizokami Hiroshi
Shiosaki Kou-ichi
Sugahara Keishin
Juridical Foundation The Chemo-Sero-Therapeutic Research Institu
Kepplinger Esther M.
Wieder Stephen C.
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