Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1985-12-20
1989-06-13
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 5, 435 68, 435235, 435239, 436820, 935 68, 935 69, C12P 2100, G01N 33576
Patent
active
048392774
ABSTRACT:
A method for the purification of HBc antigen, particularly HBc antigen produced by recombinant organisms by means of DNA recombination technique, which comprises subjecting a solution containing HBc antigen to acid-treatment, wherein the solution containing HBc antigen is acidified to a pH range of not higher than 6 by adding an acid and then resulting precipitates of lipid and contaminant proteins are removed, and then subjecting the acid-treated solution containing HBc antigen to an ion exchange chromatography with an anion exchanger, and a method for measuring HBc antibody by using said purified HBc antigen in a passive hemagglutination method, EIA method or RIA method.
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Hoofnagle et al., N. Engl. J. Med., 298:1379-1383, 1978.
Heftmann, E. (ed.), "Chromatography of Proteins," Chromatography, chapter , pp. 378-427 (1961).
Pharmacia Fine Chemicals booklet "Ion Exchange Chromatography Principles and Methods," pp. 27-54 (3/1980).
Stahl et al., "Hepatitis B Virus Core Antigen: Synthesis in Escherichia Coli and Application in Diagnosis," PNAS, vol. 79, pp. 1606-1610, Mar. 1982.
Fujita Haruo
Hamada Fukusaburo
Imamura Takayuki
Ohtomo Nobuya
Sugahara Keishin
Juridical Foundation The Chemo-Sero-Therapeutic Research Institu
Spiegel Carol A.
Warden Robert J.
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