Method for purification of cardiac troponin I

Details Method for purification of cardiac troponin I Method for purification of cardiac troponin I

1994-08-26

1996-10-01

Sayala, Chhaya D.

Drug, bio-affecting and body treating compositions

Extract, body fluid, or cellular material of undetermined...

Cardiovascular system, other than umbilical artery or vein

514 2, 514 21, 530350, 530412, 530414, 530417, 530814, A61K 3534

Patent

active

055609376

ABSTRACT:
A method is provided for isolating substantially intact cardiac troponin I from cardiac tissue comprising extracting the troponin I and purifying it in the presence of an effective mount of a mixture of protease inhibitors. The human cardiac troponin I, prepared by the present method is characterised by a molecular weight of about 28 kDa.

REFERENCES:
Rosenthal et al., Biochemical Pharmacology 42(3):685-692 (1991).
Jin et al., J. Biol. Chem. 263(15):7309-7315 (1988).
Beier et al., Eur J. Biochem. 176:327-334 (1988).
Tang et al., Shengwn Huaxue Zazhi 8(3):267-71 (1992) (Abstract).
Sugita et al., Muscle Nerve 3(4):335-339 (1980).

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