Method for purification and recovery of urokinase

Details Method for purification and recovery of urokinase Method for purification and recovery of urokinase

1976-05-04

1977-03-01

Shapiro, Lionel M.

Textiles: fiber preparation

C07G 702

Patent

active

040100740

ABSTRACT:
Highly purified urokinase is obtained in a high yield by adsorbing urokinase from an aqueous crude urokuinase solution including human urine at a pH of about 4.0 to about 7.0 on a water-insoluble polysaccharide containing agarose and agaropectin, for example, agar and Sepharose, and by eluting urokinase with an alkaline solution (pH of about 8 to 11) or concentrated salt solution.

REFERENCES:
patent: 3723251 (1973-03-01), Ogawa et al.
patent: 3746622 (1973-07-01), Nishikawa et al.
Shiba et al., Toho Igakkai Zasshi (Journal Medical Society Toho, Japan), 1973, 20(3.4)280-284; Abstracted in Chemical Abstracts vol. 80, 56897s.

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