Method for protein N-myristoylation

Chemistry: molecular biology and microbiology – Vector – per se

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435 691, 435193, 43525233, 536 232, 935 10, 935 29, 935 73, C12N 1570, C12N 1568, C12N 1554, C12N 910

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active

055040080

ABSTRACT:
There is disclosed a method for providing for the coexpression of N-myristoyltransferase and a protein substrate for said N-myristoyltransferase in E. coli comprising introducing into E. coli a dual plasmid system comprising (A) the isopropyl-.beta.-D-thiogalactopyranoside-inducible tac promoter, the g10-L ribosome binding site, a NMT gene, the kanamycin resistance gene and the p15A origin of replication in operable sequence and (B) the recA promoter, the g10-L ribosome binding site, a mammalian gene, the ampicillin resistance gene and the Col E1 origin of replication in operable sequence. This allows production of mammalian N-myristoylproteins or proteins containing covalently linked analogs of myristate with altered physical-chemical properties.

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