Chemistry: molecular biology and microbiology – Vector – per se
Patent
1994-07-13
1996-04-02
Wax, Robert A.
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 435193, 43525233, 536 232, 935 10, 935 29, 935 73, C12N 1570, C12N 1568, C12N 1554, C12N 910
Patent
active
055040080
ABSTRACT:
There is disclosed a method for providing for the coexpression of N-myristoyltransferase and a protein substrate for said N-myristoyltransferase in E. coli comprising introducing into E. coli a dual plasmid system comprising (A) the isopropyl-.beta.-D-thiogalactopyranoside-inducible tac promoter, the g10-L ribosome binding site, a NMT gene, the kanamycin resistance gene and the p15A origin of replication in operable sequence and (B) the recA promoter, the g10-L ribosome binding site, a mammalian gene, the ampicillin resistance gene and the Col E1 origin of replication in operable sequence. This allows production of mammalian N-myristoylproteins or proteins containing covalently linked analogs of myristate with altered physical-chemical properties.
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Duronio Robert J.
Gordon Jeffrey I.
Olins Peter O.
Meyer Scott J.
Moore William W.
Washington University
Wax Robert A.
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