Method for protein N-myristoylation

Details Method for protein N-myristoylation Method for protein N-myristoylation

1993-07-12

1995-07-25

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 15, 4351723, 435193, 43525233, 4353201, 935 60, 935 29, 935 42, 935 49, C12P 2100, C12N 1563, C12N 1554, C12N 1570

Patent

active

054361389

ABSTRACT:
There is disclosed a method for providing for the coexpression of N-myristoyltransferase and a protein substrate for said N-myristoyltransferase in E. coli comprising introducing into E. coli a dual plasmid system comprising (A) the isopropyl-.beta.-D-thiogalactopyranoside-inducible tac promoter, the g10-L ribosome binding site, a NMT gene, the kanamycin resistance gene and the p15A origin of replication in operable sequence and (B) the recA promoter, the g10-L ribosome binding site, a mammalian gene, the ampicillin resistance gene and the Col E1 origin of replication in operable sequence. This allows production of mammalian N-myristoylproteins or proteins containing covalently linked analogs of myristate with altered physical-chemical properties.

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