Method for protein and peptide sequencing using derivatized glas

Details Method for protein and peptide sequencing using derivatized glas Method for protein and peptide sequencing using derivatized glas

1988-08-05

1991-02-12

Warden, Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436178, 435 6, G01N 118, C12Q 168

Patent

active

049923838

ABSTRACT:
Peptides or proteins are sequenced by stepwise degradation while immobilized on a glass support derivatized with a silica-binding substance bearing a free acid group, especially a sulfonic acid group. The support is preferably derivatized with 2-(4-chlorosulfonyl phenyl) ethyl trimethoxysilane.
Peptide sequencing performance is improved if the support is also derivatized with a monomeric silica-binding substance bearing a free quaternary ammonium group, such as N-trimethyoxysilyl propyl -N,N,N-trimethyl ammonium chloride.

REFERENCES:
patent: 4603114 (1986-07-01), Hood et al.
patent: 4748121 (1988-05-01), Beaver et al.
patent: 4833093 (1989-05-01), Malmqvist et al.
Meuth et al., "Stepwise Sequence Determination from the Carboxy Terminus of Peptides," Biochemistry, 1982, vol. 21, pp. 3750-3757.
Laursen et al., "Solid Phase Methods in Protein Sequence Analysis," Methods of Biochemical Analysis, vol. 26, John Wiley & Sons, Inc: 1980, pp. 202-215.

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