Method for proliferating a liver cell, a liver cell obtained...

Details Method for proliferating a liver cell, a liver cell obtained... Method for proliferating a liver cell, a liver cell obtained...

2006-04-25

2006-04-25

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

C435S462000, C435S370000

Reexamination Certificate

active

07033744

ABSTRACT:
The present invention provides a method comprising a step of transferring a cell proliferation factor gene into a mammalian liver cell to obtain an immortalized liver cell, a step of proliferating the immortalized liver cell, and a step of removing the cell proliferation factor gene from the immortalized liver cell; a large number of liver cells obtained thereby; and a treating agent and a artificial liver comprising obtained liver cell.

REFERENCES:
J. Nakayama, “Telomerase activation by hTRT in human normal fibroblasts and hepatocellular carcinomas”,Nature Genetics, vol. 18 (Jan. 1998) pp. 65-68.
K. Nagao, “Telomerase reverse transcriptase mRNA expression and telomerase activity in hepatocellular carcinoma”,Journal of Gastroenterology(1999) pp. 83-87.
K.A. Westerman, et al; “Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination”; Proc. Natl. Acad. Sci. USA; vol. 93, pp. 8971-8976; Aug. 1996; Cell Biology.
Jin Cai, et al; “Construction of a non-tumorigenic rat hepatocyte cell line for transplantation: reversal of hepatocyte immortalization by site-specific excision of the SV40 T antigen”; Journal of Hepatology 2000; 33: 701-708.
Naoya Kobayashi, et al; “Efficient Cre/loxP Site-Specific Recombination in a HepG2 Human Liver Cell Line”; Cell Transplantation, vol. 9, pp. 737-742, 2000.

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